WebProduct Number 201064. Cell-ID Cisplatin specifically identifies dead cells when incubated prior to fixation or identifies total cells when incubated following cell fixation and permeabilization. Containing a mixture of naturally occurring platinum isotopes, it is … WebJun 19, 2024 · Cisplatin, a chemotherapeutic agent, previously employed to specifically label dead cells for mass cytometry 4 contains a platinum atom which is found as six naturally occurring isotopes with 194 ...
Alternative methods of viability determination in single …
WebMay 31, 2024 · Conclusion: CyTOF is therefore a powerful and promising method to quantify cisplatin accumulation that may be used in the clinical setting to improve and personalize cancer treatment. Keywords: ABCC2 aurones cisplatin resistance cyTOF inhibitors mass cytometry multidrug resistance ovarian cancer WebJul 20, 2024 · We also show that in an aggressive and metastatic cisplatin-resistant variant (EMT6-CDDP), CTX140 1q6d is superior and invokes an influx of intra-tumoral CD4+ and CD8+ T cells. ... (CyTOF) was ... symphony ashbourne kitchen
Platinum-conjugated antibodies for application in mass …
WebNational Center for Biotechnology Information WebMar 9, 2024 · Fc receptor blocking was performed using rat serum and dead cells were excluded using Fixable Viability Dye (flow, eBioscience) or cisplatin (CyTOF, DVS Sciences). After washing, 3 × 10 6 cells were labeled with surface markers using direct fluorochrome conjugates or biotinylated antibodies, and a streptavidin-conjugated … WebJul 25, 2024 · The first platinum anticancer drug cisplatin is widely used to treat various types of cancers including ovarian, testicular, bladder, colorectal, lung, and head and neck cancers [ 16, 17 ]. Cisplatin crosslinks with purine bases on the DNA, interfering with DNA repair mechanisms, causing DNA damage and inducing apoptosis [ 16 ]. thai anaheim ca