WebABSTRACT: The optochin susceptibility test is a key method for differentiating Streptococcus pneumoniae from other α-hemolytic streptococci; however, optochin-resistant (Optr) S. pneumoniae have been reported in the last two decades. WebThe bacitractin and optochin sensitivity tests identify whether an organism is susceptible or resistant to optochin and bacitracin. A blood agar plate is streaked for confluent growth …

Optochin Susceptibility Test: Principle, Objective, Procedure And R…

WebSpread bacteria around in a circle at least the size of a 50¢ piece. Repeat step 2 with the remaining 3 bacterial isolates. Place a taxos P disc (impregnated with optochin) on the … shanghai inquinamento

D. A Musher, “Streptococcus pneumoniae,” In G. L. Mandell, J. E ...

WebPrinciple of Optochin Susceptibility Test Ethylhydrocupreine hydrochloride (Optochin), a quinine derivative, selectively inhibits the growth of S. pneumoniae at a very low … α-hemolytic Streptococci produce colonies with the green-colored zone of incomplete hemolysis in a blood agar medium. So, it is difficult to identify S. pneumoniae among … See more Streptococcus pneumoniaeATCC 49619 produces a well-defined zone of inhibition of ≥14 mm around the optochin disk. Streptococcus mitis ATCC 49456 or Enterococcus … See more Zone size ≥14 mm = Optochin Sensitive Zone size <14 mm = Optochin Intermediate No zone of inhibition = Optochin Resistant 1. If the bacteria is Gram-positive cocci in pair, catalase-negative, and α … See more WebSusceptibility to optochin has been a mainstay for the identification of pneumococci due to the ease of performance of the test. The basis for this test is the inhibition by optochin of … shanghai institute of biological products